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Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity

CRISPR–Cas9 is a powerful genome-editing tool, but genome-wide off-target activity can hinder therap...

SCIENCE March 25, 2026

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity

CRISPR–Cas9 is a powerful genome-editing tool, but genome-wide off-target activity can hinder therapeutic applications. Negative supercoiling ((−)SC) has...

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CRISPR–Cas9 is a powerful genome-editing tool, but genome-wide off-target activity can hinder therapeutic applications. Negative supercoiling ((−)SC) has...

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